Human Lung Lym Endothelial Cells

Description

Human Lung Lym Endothelial Cells (HLyECs) are primary human microvascular endothelial cells isolated from the lung, specifically enriched from pulmonary lymphatic vessels of healthy donors. These specialized endothelial cells exhibit characteristic cobblestone monolayer morphology and maintain critical lymphatic functions including pulmonary lymph drainage regulation, immune cell trafficking to bronchial lymph nodes, and interstitial fluid balance. These cells serve as a valuable and authentic in vitro model for pulmonary endothelial research, owing to their specialized pulmonary lymphatic endothelial properties and intimate functional connections with the pulmonary vascular system. Their dysfunction contributes to acute respiratory distress syndrome (ARDS), pulmonary edema, and tumor metastasis via lung lymphatics. The cells are positive for lymphatic-specific markers PROX1, LYVE-1, and VEGFR3. As primary cells, they have limited proliferative capacity and require specialized media. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.