Human Mesothelial Cells, Adult, Diabetic Donor

Description

Human Mesothelial Cells, Adult, Diabetic Donor are primary cells isolated from pleural or peritoneal tissue of adult donors with clinically confirmed Type 1 or Type II diabetes Mellitus (T1DM/T2DM). These cells exhibit characteristic cobblestone morphology in adherent monolayers. Functionally compromised by hyperglycemia, they demonstrate disease-specific conditions including impaired barrier integrity, altered hyaluronan secretion, and dysregulated inflammatory responses. The cells show the expression of mesothelial markers calretinin and podoplanin alongside pathological overexpression of diabetic markers RAGE. Cryopreserved at early passage, these cells maintain authentic hyperglycemia-induced phenotypes but show severely limited proliferation. To preserve optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.