Human Ovarian Microvascular Endothelial Cells

Description

Human Ovarian Microvascular Endothelial Cells (HOMEC ) are derived from healthy human ovarian tissue. Following the primary culture, these cells are cryopreserved. Human Ovarian Microvascular Endothelial Cells are involved in the regulation of vascular tone, maintenance of selective vascular permeability, and mediation of leukocyte trafficking. Studies have shown that these cells are closely associated with various physiological and pathological processes, including angiogenesis and inflammatory responses. These cells express a range of endothelial-specific markers, including von Willebrand factor (vWF), Ulex europaeus agglutinin I (UEA-1), CD31, and E-selectin. In addition, they express VCAM-1, a marker indicative of activated endothelial cells. RT-PCR analysis reveals the presence of vascular endothelial growth factor receptors (flt-1 and KDR) and endothelial nitric oxide synthase (eNOS). In vitro, repeated freezing and thawing of these cells should be avoided during culture.

Application

• Human Ovarian Microvascular Endothelial Cells serve as an excellent in vitro model for studying angiogenesis, particularly within the microvascular endothelium. Angiogenesis plays a vital role in various physiological processes, including organ development and tissue repair. These cells are especially useful for research into the angiogenic mechanisms within the ovary, such as the development of the corpus luteum. During this process, microvascular endothelial cells from the thecal layer invade the avascular granulosa layer to establish a capillary network, making these cells essential for investigating ovarian function and vascular dynamics in reproductive biology.