Human Renal Epithelial Cells

Description

Human Renal Epithelial Cells are isolated from whole human kidneys of healthy donors. These cells are a type of primary epithelial cells, showing polygonal or cuboidal morphology and an adherent growth pattern. They are cyropreserved at early passages to maintain high viability and functionality. Human renal epithelial cells play a key role in kidney tissue morphogenesis and repair, notably responding to acute kidney injury (AKI) by rapid proliferation. They express epithelial markers such as cytokeratin 18 and 19. Their karyotype is human diploid (46,XX or 46,XY), indicating genomic stability. Tthey are ideal for studies on renal disease models, drug toxicity screening, ischemic injury, and tissue regeneration.

Application

• Research on human renal epithelial cells is essential for bioartificial kidney engineering strategies. The polarization of human renal epithelium is originated from integrin-mediated cell-matrix adhesion, which responds to matrix stiffness, as shown by cellular mechanobiology studies. This suggests matrix stiffness could be used to regulate proliferative cells. In the heterogeneous epithelial cells, renal tubule epithelial cells express toll-like receptors (TLRs) and may respond to bacterial infection and ischemic stress via innate immunity.The role of TLRs in the kidney could be further investigated using human renal epithelial cells.