Human Renal Proximal Tubule Cells

Description

Human Renal Proximal Tubule Cells (RPTCs) are primary epithelial cells isolated from the proximal convoluted segment of normal adult human kidneys. These cells are cryopreserved at early passage. In vitro, RPTCs display characteristic epithelial features, such as a spread-out monolayer, and retain their functional properties under specific culture conditions. These kidney cells form a polarized monolayer and play essential roles in reabsorbing glucose, amino acids, sodium, and bicarbonate, while secreting waste and xenobiotics. These renal tubular cells express renal markers like γ-glutamyl transpeptidase, alkaline phosphatase, megalin, and transporters (OAT1, OCT2), important for nephrotoxicity studies. They are sensitive to toxins and ischemia, making them a crucial in vitro model for kidney function and disease research.

Application

• Human renal proximal tubule cells (HRPTCs) play a crucial role in renal homeostasis, making them a valuable in vitro model for studying renal tubular cell biology and pathophysiology, including their repair mechanisms after injury, their contribution to renal fibrosis progression, intercellular communication with other cells, high-throughput drug and toxicity screening, and their involvement in renal diseases such as tubular necrosis, tubulointerstitial injury, nephrotoxicity, and renal cancer.