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Primary Cells

Human Small Intestine Epithelial Cells

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Human Intestinal Epithelial Cells were initiated by elutriation of dispase dissociated normal human small intestine tissue. These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell […]
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Home | Primary Cells | Human Primary Cells | Digestive System Primary Cells | Human Small Intestine Epithelial Cells
Species

Human
Cat.No

ABC-TC3814
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Intestine
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Intestinal Cells
Key Features

– Backed by AcceGen advanced technology
– Cryopreserved for highest viability and plating efficiency
– Quality-tested for accurate results
– Expert teams to provide timely support and assist best experimental outcomes

Description

Human Small Intestine Epithelial Cells are isolated from human small intestinal tissue. The cells are epithelial-like, polygonal, and grow in an adherent manner. These cells are located on the surface of the intestinal cavity and are the main site for food digestion and absorption. They play a key role in nutrient absorption, secretion, and motility. They are also the first line of defense for the body against pathogenic microorganisms and play a key role in the initial stage of mucosal immune response. Intestinal crypts and villi are structures within the intestinal epithelium that contain various specialized epithelial cell lineages, including absorptive enterocytes, enteroendocrine cells, goblet cells, and Paneth cells.They express epithelial markers such as cytokeratin 18 (CK18) and tight junction proteins like zonula occludens-1 (ZO-1), detectable by immunocytochemistry.

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Citation

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Application

  • Human Small Intestine Epithelial Cells can be used to study intestinal absorption and transport mechanisms. These cells can also be used to explore the pathogenesis of diseases such as inflammatory bowel disease (IBD) and leaky gut syndrome, and to test potential therapeutic strategies (such as tight junction regulators).

Frequently Asked Questions

  • What are Human Small Intestine Epithelial Cells?

    Human Small Intestine Epithelial Cells are cells that line the surface of the small intestine, responsible for absorbing nutrients, secreting enzymes, and forming a barrier against pathogens and toxins.

  • How many times can Human Small Intestine Epithelial Cells be passaged in culture?

    Human Small Intestine Epithelial Cells can typically be passaged 3-5 times before their characteristics and functionality begin to diminish. Researchers often aim to use cells within a limited number of passages to ensure reliable and reproducible results in experiments.

  • What functions do Human Small Intestine Epithelial Cells perform in the small intestine?

    Human Small Intestine Epithelial Cells are involved in nutrient absorption (such as carbohydrates, proteins, and lipids), secretion of digestive enzymes and mucus, maintaining the intestinal barrier, and interacting with gut-associated lymphoid tissues to regulate immune responses.

  • Where are Human Small Intestine Epithelial Cells isolated from?

    Human Small Intestine Epithelial Cells are isolated from the small intestine, which includes the duodenum, jejunum, and ileum.

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