Human Umbilical Vein Smooth Muscle Cells

Description

Human Umbilical Vein Smooth Muscle Cells (HUVSMCs) are primary smooth muscle cells isolated from the media of the human umbilical vein. These cells exhibit a spindle-shaped or elongated morphology, typical of smooth muscle cells (SMCs), with a central nucleus and a contractile phenotype under normal culture conditions. HUVSMCs play a critical role in maintaining vascular tone, regulating blood flow via contraction and relaxation, and participating in vascular repair and remodeling. HUVSMCs express key SMC markers, including alpha-smooth muscle actin (α-SMA), calponin, smooth muscle myosin heavy chain (SM-MHC), and transgelin/SM22α, which are essential for their contractile function and structural integrity. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.