For research use only
| Cat No. | ABC-TC0463 |
| Product Type | Mouse Sarcoma Cell Lines |
| Species | Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Blood |
| Disease | Sarcoma |
| Product Code | J-774A.1; J774.A1; J774 A1; J774A1; J 774A.1; J774 A.1 |
J774A.1 Cells are active in antibody dependent phagocytosis [Pubmed: 1101071].
J774A.1 is a murine monocyte/macrophage-like tumor cell line originally derived from the ascites tumor of a female BALB/c mouse with reticulum cell sarcoma. It exhibits adherent to semi-adherent macrophage morphology in culture, with a granular cytoplasm and macrophage-typical behavior such as antibody-dependent phagocytosis. J774A.1 cells synthesize and continuously secrete interleukin-1 (IL-1) and lysozyme, and can produce other cytokines (e.g., IL-5) and growth factors. The line expresses receptors for immunoglobulin and complement, and supports studies of innate immune activity and macrophage responses. J774A.1 is widely used for macrophage-centric functional studies. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.
| Product Code | J-774A.1; J774.A1; J774 A1; J774A1; J 774A.1; J774 A.1 |
| Species | Mouse |
| Cat.No | ABC-TC0463 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Growth Mode | Semi-adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Blood |
| Disease | Sarcoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Sarcoma Cell Lines |
J774A.1 mouse macrophages serve as an in vitro model of murine macrophages for investigating innate immune responses, phagocytosis, cytokine production, and inflammatory signaling pathways. They are applied in immunology research, metabolic studies, pathogen-host interaction assays, and pharmacological evaluations of compounds affecting macrophage function.
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J774A.1 cells were isolated from the ascitic fluid of an adult female BALB/cN mouse with reticulum cell sarcoma. J774A.1 cells exhibit antibody-dependent phagocytosis and their growth is inhibited by dextran sulfate, PPD and LPS. J774A.1 cells can produce IL-1β and large amounts of lysozyme.
Cells are semi-adherent, subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Do not use trypsin. On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet, then re-suspended in fresh media. The J774A.1 seeding density is 2-4 x 10⁴/cm2, cells will show signs of re-attachment within 48 hours.
It seem so. The J774 mouse macrophages seem more susceptible to the toxic effects of oritavancin than human THP-1 cells. This is likely because J774 cells accumulate the drug to a greater extent than human cells.
