Monkey Liver Fibroblasts

Description

Monkey Liver Fibroblasts (MLFs) are primary fibroblasts isolated from liver tissue of the Cynomolgus Monkey. These cells represent liver-derived mesenchymal fibroblasts that typically display spindle-shaped or stellate morphology with elongated cytoplasmic processes and exhibit adherent growth characteristics under standard culture conditions. They can generally be expanded in vitro for approximately 3–6 passages at a split ratio of 1:3 under the cell culture conditions specified by AcceGen. Liver fibroblasts are important stromal cells involved in maintaining hepatic tissue architecture and extracellular matrix (ECM) homeostasis. They contribute to the synthesis and remodeling of ECM components and participate in tissue repair and wound-healing processes within the liver microenvironment. MLFs typically express fibroblast-associated markers such as Vimentin. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.