| Species |
Mouse
|
| Cat.No |
ABC-TC142W
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Primary Cells |
| Size/Quantity |
1 vial
|
| Cell Type |
Epithelial Cell
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 °C, 5% CO2
|
| Source Organ |
Epididymis
|
| Disease |
Normal
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Epididymal Cells
|
Description
Mouse Epididymal Epithelial Cells (MEECs) are primary epithelial cells isolated from the caput region of adult mouse epididymis using enzymatic digestion and selective culture conditions. These cells maintain a polarized pseudostratified morphology, express tight junction proteins (e.g., claudin‑1, occludin) and transporters such as monocarboxylate transporter 1 (MCT1), facilitating luminal acidification and nutrient exchange. MEECs also possess primary cilia that detect fluid shear stress and initiate intracellular calcium signaling and cytoskeletal remodeling. Furthermore, MEECs express pattern recognition receptors (PRRs) including Toll‑like receptor 3 (TLR3), triggering type I interferon, tumor necrosis factor‑α (TNF‑α), and monocyte chemoattractant protein‑1 (MCP‑1) secretion upon viral mimic stimulation. Therefore, MEECs provide a reliable in vitro model for studying barrier integrity, mechanosensing, innate immunity, and sperm–epithelial interactions.
