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| Species | Rat |
| Cat.No | ABC-TC5569 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Lymphoblast |
| Shipping Info | Dry ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Thymus |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Rat Lymphoma Cell Lines |
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For research use only
Nb2-11 cells are lymphoblastoid cells which grow in suspension and clump to form clusters when they are close to full confluence.
The culture medium recommended for Nb2-11 cells is composed of Fischer’s medium supplemented with 0.075% Na Bicarbonate, 2mM Glutamine to support optimal cell growth and maintenance, add 0.05mM 2-Mercaptoethanol (2ME), 10% FBS and 10% HS. Nb2-11 cell culture does not have to use HEPEs.
It is recommended to seed at 4×1,000 and 12×1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9×100,000 cells/ml. Check daily.
Nb2-11 is a clone of the Nb-2 rat lymphoma line, derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment.
