Explore Products
Tumor Cell Lines

NCI-H322

  • 362
NCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol […]
Request Your Custom Quote Today

Discover top-quality products tailored for scientific and medical research. Request a personalized quote today
to enhance your projects.

Get a Quote
  • High Purity Levels
  • Precision and Reliability
  • Customization Options
Product Code

H322; H-322; H322T; NCI-H322T; NCIH322T; NCI-322; NCIH322

Species

Human

Cat.No

ABC-TC5561

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Shipping Info

Dry ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Lung

Disease

Lung Adenocarinoma

Storage

Liquid Nitrogen

Product Type

Human Lung Cancer Cell Lines

Description

NCI-H322
The NCI-H322 cell line is a human epithelial tumor cell line derived from a 52-year-old Caucasian male with primary bronchioalveolar carcinoma, a non-small cell lung cancer. The cells display epithelial morphology with Clara cell-like ultrastructural characteristics and grow as adherent monolayers under standard conditions (37°C, 5% CO₂). They are tumorigenic in athymic mice. NCI-H322 cells express glutathione-S-transferase, phenol sulfotransferase, and surfactant protein A (SP-A), but lack UDP-glucuronosyltransferase expression. They are ideal for investigating carcinogen metabolism, specifically benzo(a)pyrene (BaP), and their cytotoxic response to BaP suggests involvement of metabolic products like 7,8-diol-BaP. NCI-H322 cells are widely used in lung carcinogenesis, SP-A regulation, and preclinical drug testing targeting tumor suppressor genes and anti-cancer pathways.
View Product Image

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • The NCI-H322 cell line, due to its deficient glucuronosyltransferase activity, presents an opportunity to investigate the physiological impacts of toxic chemicals that are typically detoxified through glucuronic acid conjugation. These cells serve as valuable test systems for assessing the cytotoxicity and genotoxicity of various substances on the human lung. Moreover, this cell line plays a crucial role in studying genes as tumor suppressors in the development of human lung cancer and assessing the effectiveness of potential drugs or inhibitors for their anti-tumor activity, frequently involving murine models. Overall, NCI-H322 cells have diverse applications in toxicology, lung carcinogenesis research, and preclinical drug testing.

Frequently Asked Questions

  • What are NCI-H322 cells?

    NCI-H322 is a human non-small cell lung cancer (NSCLC) cell line used in cancer research.

  • Where can I obtain NCI-H322 cells?

    NCI-H322 cells can be obtained from cell line repositories such as the ACCEGEN (ABC-TC5561 NCI-H322).

  • What type of culture vessels should be used for NCI-H322 cells?

    Use tissue culture-treated flasks or dishes to ensure proper cell adherence.

  • What should I do if my NCI-H322 cells are not proliferating?

    Check Culture Conditions: Ensure the temperature, CO₂ levels, and medium are appropriate.

     

    Serum Concentration: Verify that the FBS concentration is at 10%.

     

    Contamination Check: Inspect for contamination and treat or discard contaminated cultures.

     

    Cell Viability: Consider using fresh cells or a new batch.

Inquiring NCI-H322

We know how valuable your research is to you, but are you wondering what you can expect to pay for quick accurate results every time? Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

Tags

AcceGen Scroll Top Button