PF-382

PF-382 is a human T-cell acute lymphoblastic leukemia (T-ALL) cell line established from the pleural effusion of a 6-year-old female patient. The cells exhibit suppressive phenotypic and functional characteristics. Morphologically, PF-382 cells are single, round to oval in shape, and grow in suspension culture. Cytogenetic analysis reveals a modal chromosome number of 46 with a translocation between chromosomes X and 15 (46X,Xq–,15p+). Immunophenotyping shows expression of early T-lymphocyte markers OKT6, Leu-1, Leu-9, and the mature marker OKT8. The cell line lacks CD3 expression and does not form rosettes with sheep erythrocytes. PF-382 cells secrete a 47 kDa inhibitory factor that suppresses the proliferation of myeloid and erythroid progenitor cells. Genetic analyses have identified mutations in several oncogenes and tumor suppressor genes, including TAL1, CREBBP, HRAS, JAK1, NOTCH1, NRAS, PTEN, and TP53.  The tumorigenic potential of PF-382 in vivo has not been characterized.