Rat Kidney Fibroblasts

Description

Rat Kidney Fibroblasts (RKF) are primary cells isolated from the interstitial connective tissue of healthy rat kidneys. These cells exhibit a spindle-shaped morphology characteristic of fibroblasts and adhere firmly to standard tissue culture-treated surfaces. In vitro, they form elongated, contact-inhibited monolayers and exhibit robust proliferation under optimized culture conditions. Rat Kidney Fibroblasts express classical mesenchymal markers such as vimentin, fibronectin, and α-smooth muscle actin (α-SMA), particularly under profibrotic stimulation. They are cryopreserved at early passage to ensure phenotypic stability and optimal post-thaw viability. To ensure optimal cellular function and reproducibility, repeated freeze-thaw cycles are not recommended. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.