For research use only
| Cat No. | ABC-TC1006 |
| Product Type | Human Bone Cancer Cell Lines |
| Cell Type | Epithelial-like |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Bone |
| Product Code | SAOS-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS |
Saos-2 is a human osteosarcoma cell line with osteoblastic traits, producing bone proteins and responding to hormones, used for bone research.
Saos-2 is a human osteosarcoma cell line derived from the primary tumor of an 11-year-old Caucasian female. These cells exhibit epithelial-like morphology and grow as an adherent monolayer with a doubling time of approximately 48 hours. SaOS‑2 cells also display an aneuploid karyotype (~56 chromosomes, with complex rearrangements), and are deficient in both TP53 and RB1 tumor suppressor pathways. Saos-2 demonstrate key osteoblastic properties, including high alkaline phosphatase (ALP) activity and production of bone-specific proteins such as osteocalcin and osteopontin. They also express functional receptors for 1, 25-dihydroxyvitamin D3 and parathyroid hormone (PTH), enabling cAMP-mediated responses upon hormonal stimuli. They possess osteoblastic characteristics including the ability to deposit a mineralized extracellular matrix and differentiate toward osteocyte-like phenotypes under mineralizing conditions, with upregulated expression of late markers such as SOST, DMP1, PHEX, and MEPE. Although non-tumorigenic in routine xenograft assays, they form mineralized tissue when cultured in diffusion chambers implanted in mice. The line remains highly transfectable and phenotypically robust for bone biology and osteosarcoma research.
| Product Code | SAOS-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS |
| Species | Human |
| Cat.No | ABC-TC1006 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial-like |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Bone |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Bone Cancer Cell Lines |
The Saos-2 cell line has served as a pivotal model system in bone cancer research for decades, providing unique insights into late-stage osteoblastic differentiation. Despite their osteosarcoma origin, Saos-2 cells exhibit a remarkably mature osteoblast phenotype characterized by elevated alkaline phosphatase (ALP) activity and other differentiation markers, making them an exceptional in vitro model for studying normal osteoblast biology. In comparative analyses with other osteosarcoma cell lines and primary human osteoblasts, Saos-2 cells consistently demonstrate the most advanced osteoblastic differentiation profile, underscoring their utility for investigating molecular mechanisms of osteoblast maturation.
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