For research use only
| Cat No. | ABC-RC034Y |
| Product Type | Reporter Stable Cell Lines |
| Cell Type | Epithelial |
| Species | Mouse |
| Host Cell | 4T1 |
| Source Organ | Breast |
| Disease | Mouse breast cancer |
| Storage | Liquid Nitrogen |
4T1/STAT5 Reporter (GFP) Stable Cell Line for real-time visualization of STAT5 activation in breast cancer metastasis and immune microenvironment studies.
4T1/STAT5 Reporter (GFP) stable Cell Line is a genetically engineered model derived from the murine 4T1 mammary carcinoma parental cell line, designed for studying STAT5 (Signal Transducer and Activator of Transcription 5) signaling pathways. This cell line exhibits adherent epithelial morphology and stably expresses a GFP (Green Fluorescent Protein) reporter under STAT5-responsive elements, enabling real-time monitoring of STAT5 activity. Key features include high GFP fluorescence upon STAT5 activation, making it suitable for drug screening and pathway analysis. The cells retain tumorigenic properties, including metastatic potential, and are validated for consistent STAT5 responsiveness. Overexpression of STAT5-regulated genes, such as Bcl-xL and Cyclin D1, can be quantified via GFP intensity. Cells are maintained at low passages (<P20) to ensure stability and performance. The cells have undergone rigorous quality control testing and are confirmed free of contamination from mycoplasma, fungi, yeast, and bacteria.
| Species | Mouse |
| Cat.No | ABC-RC034Y |
| Product Category | Transfected Stable Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Breast |
| Disease | Mouse breast cancer |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Reporter Stable Cell Lines |
| Host Cell | 4T1 |
| Gene Info | STAT5 / GFP |
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
The 4T1/STAT5 Reporter (GFP) Stable Cell Line is a murine breast cancer model engineered to monitor STAT5 signaling activity through GFP expression. This reporter system enables real-time, fluorescence-based tracking of STAT5 pathway activation in response to cytokines, growth factors, or drug treatments. Ideal for studying metastasis, tumor microenvironment interactions, and immunotherapy responses, the cell line provides quantitative readouts of STAT5 activity in both in vitro and in vivo applications. Maintain in DMEM medium with appropriate selection agents to ensure stable GFP expression throughout experiments.