Transfected Stable Cell Lines

4T1/STAT5 Reporter (GFP) Stable Cell Line

  • For research use only

Cat No.

ABC-RC034Y

Product Type

Reporter Stable Cell Lines

Cell Type

Epithelial

Species

Mouse

Host Cell

4T1

Source Organ

Breast

Disease

Mouse breast cancer

Storage

Liquid Nitrogen

4T1/STAT5 Reporter (GFP) Stable Cell Line for real-time visualization of STAT5 activation in breast cancer metastasis and immune microenvironment studies.

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Description

4T1/STAT5 Reporter (GFP) stable Cell Line is a genetically engineered model derived from the murine 4T1 mammary carcinoma parental cell line, designed for studying STAT5 (Signal Transducer and Activator of Transcription 5) signaling pathways. This cell line exhibits adherent epithelial morphology and stably expresses a GFP (Green Fluorescent Protein) reporter under STAT5-responsive elements, enabling real-time monitoring of STAT5 activity. Key features include high GFP fluorescence upon STAT5 activation, making it suitable for drug screening and pathway analysis. The cells retain tumorigenic properties, including metastatic potential, and are validated for consistent STAT5 responsiveness. Overexpression of STAT5-regulated genes, such as Bcl-xL and Cyclin D1, can be quantified via GFP intensity. Cells are maintained at low passages (<P20) to ensure stability and performance. The cells have undergone rigorous quality control testing and are confirmed free of contamination from mycoplasma, fungi, yeast, and bacteria.

Species

Mouse

Cat.No

ABC-RC034Y

Product Category

Transfected Stable Cell Lines

Size/Quantity

1 vial

Cell Type

Epithelial

Growth Mode

Adherent

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Breast

Disease

Mouse breast cancer

Biosafety Level

2

Storage

Liquid Nitrogen

Product Type

Reporter Stable Cell Lines

Host Cell

4T1

Gene Info

STAT5 / GFP

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • The 4T1/STAT5 Reporter (GFP) Stable Cell Line is a murine breast cancer model engineered to monitor STAT5 signaling activity through GFP expression. This reporter system enables real-time, fluorescence-based tracking of STAT5 pathway activation in response to cytokines, growth factors, or drug treatments. Ideal for studying metastasis, tumor microenvironment interactions, and immunotherapy responses, the cell line provides quantitative readouts of STAT5 activity in both in vitro and in vivo applications. Maintain in DMEM medium with appropriate selection agents to ensure stable GFP expression throughout experiments.

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

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