B6129SF2/J Mouse Cardiac Fibroblasts

Description

B6129SF2/J Mouse Cardiac Fibroblasts (CFs) are primary cells isolated from cardiac tissue of B6129SF2/J mice. These cells constitute over 90% of non-myocardial cells in the heart, exhibiting spindle-shaped or polygonal morphology with large oval nuclei and no spontaneous beating. CFs are adherent and proliferate rapidly but have limited passaging capacity. Functionally, CFs synthesize extracellular matrix (ECM) components (e.g., collagen, elastin) to support cardiomyocyte structure and secrete paracrine growth factors during cardiac injury, providing a relevant model for studying B6129SF2/J cardiac cell interaction. Upon activation (e.g., post-myocardial infarction), they differentiate into myofibroblasts expressing α-smooth muscle actin (α-SMA) and collagen I. Expression of α-SMA and collagen I is confirmed by immunocytochemistry. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.