B6129SF2/J Mouse Liver Cells

Description

B6129SF2/J Mouse Liver Cells are isolated from the hepatic tissue of B6129SF2/J mice, encompassing a heterogeneous population including hepatocytes and non-parenchymal liver cells, and are suitable for studies of B6129SF2/J mouse liver physiology. These cells contribute to various physiological functions such as metabolism, detoxification, and protein synthesis. Cells are cryopreserved immediately after isolation and exhibit limited proliferative capacity in vitro. These cells display typical hepatocyte polygonal morphology with prominent nuclei in culture. In vitro, they retain liver-specific markers like albumin and cytochrome P450 enzymes, essential for xenobiotic metabolism. They respond to peroxisome proliferator-activated receptor alpha (PPARα) agonists, making them suitable for investigating toxicity pathways and metabolic regulation. Expression of albumin and cytochrome P450 enzymes is confirmed by immunostaining. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.