C-4 II

Description

C-4 II is a human cervical carcinoma cell line derived from a 41-year-old Caucasian female. These epithelial cells form monolayered colonies with distinct hemicysts, characteristic of transporting epithelia. They exhibit contact inhibition, leading to cell separation and shedding under crowded conditions. Even after extended cultivation, C-4 II cells maintain basal cell differentiation properties and demonstrate G6PD type B. Karyotypic analysis reveals a hypodiploid chromosome number with specific abnormalities, indicating genomic instability. Notably, these cells harbor integrated human papillomavirus type 18 (HPV-18) DNA sequences and actively express HPV-18 RNA. C-4 II cells are tumorigenic, forming poorly differentiated squamous carcinomas when injected subcutaneously into nude mice.

Application

• The C-4 II cell line  is widely applied across multiple research disciplines. In 3D cell culture, these cells serves as a robust model for investigating cellular behavior and tissue architecture in physiologically relevant 3D environments. In cancer research, C-4 II cells  is instrumental in exploring cervical carcinoma progression and identifying therapeutic targets. Additionally, in infectious disease research, their interaction with human papilloma virus 18 (HPV-18)provides a model to study viral-host interactions and evaluate antiviral approaches. Furthermore, in sexually transmitted disease research, studying HPV-18’s impact on these cells contributes to understanding the virus’s pathogenesis and transmission dynamics, aiding in the development of preventive measures and treatments.