C57BL/10J Mouse Stomach Epithelial Cells

Description

C57BL/10J Mouse Stomach Epithelial Cells are primary cells derived from the stomach tissue of pathogen-free C57BL/10J mice. These cells exhibit the typical cobblestone morphology of epithelial cells and are cultured in gelatin-coated T75 flasks with complete growth medium for 3–7 days. They are known to express epithelial markers such as E-cadherin and cytokeratins (e.g., CK18, CK20), supporting their gastric epithelial identity. These cells are non-passagable and are cryopreserved at passage one to ensure functional integrity. Mouse stomach epithelial cells are essential for in vitro models of gastric mucosal biology, particularly for studying cellular responses to pathogens, acidity, and inflammation, as well as the early stages of gastric cancer development. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.