C57BL/6 Mouse Colonic Smooth Muscle Cells

Description

C57BL/6 Mouse Colonic Smooth Muscle Cells (CSMCs) are primary cells isolated from the colonic tissue of pathogen-free laboratory mice, serving as a robust model for C57BL/6 colon peristalsis studies. These specialized cells originate from the muscle layers of the colon wall. Following primary culture, these cells are cryopreserved. They regulate propulsive motility through calcium-dependent contractions of their circular and longitudinal layers, coordinating peristalsis via enteric neuronal input. Studies have demonstrated their involvement in chronic constipation due to interstitial Cajal cell loss, TNBS-induced colitis-associated hypercontractility, and aganglionosis in Hirschsprung’s disease models. Mouse Colonic Smooth Muscle Cells are characterized by antibodies against α-smooth muscle actin. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.