C57BL/6 Mouse Kidney Fibroblasts

Description

C57BL/6 Mouse Kidney Fibroblasts are primary cells isolated from the kidney tissue of pathogen-free laboratory mice. These mouse fibroblasts reside in the renal interstitium, especially within the tubulointerstitial compartment surrounding renal tubules. Following primary culture, these cells are cryopreserved. The cells play a crucial role in mediating extracellular matrix remodeling, inflammatory cytokine secretion, and immune cell recruitment via chemokine signaling. Studies have demonstrated their critical involvement in chronic kidney disease, diabetic nephropathy, and obstructive nephropathy. Phenotypic characterization confirms expression of fibroblast markers CD90.2 (Thy1.2), PDGFR-β, and FSP-1 (S100A4). Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria. ​