C57BL/6 Mouse Liver Cells are primary cells isolated from the liver tissue of pathogen-free C57BL/6 mice. This heterogeneous population were characterized using immunocytochemical methods, including parenchymal hepatocytes, Kupffer cells (F4/80+ macrophages), hepatic stellate cells (GFAP+), and endothelial cells (CD34+). Functionally, Kupffer cells perform phagocytosis and immune surveillance, clearing pathogens and cellular debris, whereas stellate cells regulate extracellular matrix remodeling. These cells are implicated in liver injury models, including non-alcoholic fatty liver disease (NAFLD) and toxin-induced hepatitis through cytokine secretion (e.g., TNF-α, IL-6) and inflammatory responses. Due to limited expansion capacity, repeated freezing/thawing cycles should be avoided to maintain viability. This primary cell system provides a physiologically relevant platform for studying hepatic pathophysiology without genetic background variability .
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