C57BL/6 Mouse Liver Cells

Description

C57BL/6 Mouse Liver Cells are primary cells isolated from the liver tissue of pathogen-free C57BL/6 mice. This heterogeneous population were characterized using immunocytochemical methods, including parenchymal hepatocytes, Kupffer cells (F4/80+ macrophages), hepatic stellate cells (GFAP+), and endothelial cells (CD34+). Functionally, Kupffer cells perform phagocytosis and immune surveillance, clearing pathogens and cellular debris, whereas stellate cells regulate extracellular matrix remodeling. These cells are implicated in liver injury models, including non-alcoholic fatty liver disease (NAFLD) and toxin-induced hepatitis through cytokine secretion (e.g., TNF-α, IL-6) and inflammatory responses. Due to limited expansion capacity, repeated freezing/thawing cycles should be avoided to maintain viability. This primary cell system provides a physiologically relevant platform for studying hepatic pathophysiology without genetic background variability .

Application

• Mouse liver cells, particularly hepatocytes, hold significant value as a predictive tool in drug development. They mimic how drug candidates might interact within human livers, aiding preclinical in vitro studies focused on drug metabolism and pharmacokinetics. These cells are exceptionally useful for assessing drug clearance, metabolism, uptake, hepatotoxicity, and drug-drug interactions. Since they share analogous drug-metabolizing enzymes with human hepatocytes, particularly in the context of biotransformation, they offer a robust model for predicting human responses. Cryopreserved mouse liver cells offer an economical and practical substitute for in vivo trials, providing diverse formats tailored to individual requirements, ultimately advancing drug research and development endeavors.