C57BL/6 Mouse Lung Cells

Description

C57BL/6 Mouse Lung Cells are primary cells isolated from the lung tissue of pathogen-free laboratory mice, serving as a fundamental model for studying lung structure in mice, characterizing C57BL/6 alveolar cells, and exploring mouse respiratory epithelium. These cells reside within the tracheobronchial epithelium, distal bronchioles, alveolar septa, and alveolar lumen of the pulmonary parenchyma. Following primary culture, these cells are cryopreserved. The cells maintain critical respiratory functions including facilitating gas exchange (alveolar epithelium), airway clearance (mucociliary escalator), and immune defense (alveolar macrophage activation). Studies demonstrate their implication in idiopathic pulmonary fibrosis (TGF-β-induced EMT), asthma/COPD (Th2-mediated mucus hypersecretion), and lung adenocarcinoma. Phenotypic characterization confirms cell-type-specific markers including CC10 for Clara cells, SPC for type II pneumocytes, and CD68 for alveolar macrophages. For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.