C57BL/6 Mouse Mammary Microvascular Endothelial Cells

Description

C57BL/6 Mouse Mammary Microvascular Endothelial Cells are primary cells isolated from the mammary tissue of pathogen-free laboratory mice, serving as an important model for breast endothelial research and mouse mammary microvascular development. They are exclusively localized in the capillary networks of mammary gland stroma. Following primary culture, these cells are cryopreserved. The cells orchestrate critical physiological processes including VEGF/VEGFR2-mediated angiogenesis, VE-cadherin-regulated vascular permeability, and ICAM-1-dependent immune cell trafficking. Studies demonstrate their involvement in breast cancer, mammary fibrosis, and preeclampsia-like placental defects. Phenotypic validation confirms expression of endothelial markers VE-cadherin, von Willebrand factor (vWF), and CD31/PECAM-1. For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.