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Primary Cells

CD-1 Mouse Embryonic Fibroblasts MMC treated

  • For research use only

Cat No.

ABC-TC3281
Product Type

Mouse Primary Cells
Cell Type

Fibroblast
Species

CD-1 Mouse
Growth Conditions

37 ℃, 5% CO2
Source Organ

Embryo
Disease

Normal
Storage

Liquid Nitrogen

CD-1 Mouse Embryonic Fibroblasts MMC treated support the growth of undifferentiated ES and iPS cells, providing feeder layers for stem cell research.

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  • CD-1 Mouse Embryonic Fibroblasts MMC treated
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Home | Primary Cells | Animal Primary Cells | Mouse Primary cells | CD-1 Mouse Embryonic Fibroblasts MMC treated

Description

CD-1 Mouse Embryonic Fibroblasts (MEFs) treated with Mitomycin C (MMC) are isolated from pathogen-free CD-1 embryos via enzymatic digestion. MMC treatment arrests their proliferation, generating growth-arrested feeder cells that support the maintenance of undifferentiated mouse and human ES/iPS cells. These cells secrete extracellular matrix proteins and growth factors essential for pluripotency preservation and provide a stable substrate for stem cell adhesion and growth, and enable modeling of CD-1 fibroblast division after MMC treatment. MMC-treated MEFs are reliable feeder cells for pluripotent stem cell culture and related research applications, showing cell cycle arrest in CD‑1 mouse cells following MMC exposure. To ensure the quality of these primary cells, repeated freeze-thawcycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.

Product Code

CD-1 Mouse Embryonic Fibroblasts, Mouse Embryonic Fibroblasts MMC Treated, Embryonic Fibroblasts, Fibroblasts, MEFs, CD-1 MEFs (MMC)
Species

CD-1 Mouse
Cat.No

ABC-TC3281
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Fibroblast
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Embryo
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Mouse Primary Cells
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • MMC-treated CD-1 MEFs act as a feeder layer to maintain the undifferentiated state of pluripotent stem cells during culture. Their growth arrest prevents over-proliferation, allowing stem cells to expand without competition. These MEFs are indispensable for stem cell maintenance, differentiation studies, reprogramming protocols, and developmental biology research, providing a consistent and supportive microenvironment.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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