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Tumor Cell Lines

Hep 3B

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Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.
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Product Code

Hep 3B2_1-7; HEP3B217; Hep 3B2; HEP-3B2; HEP3B2; Hep-3B; HEP-3B; Hep 3B; Hep3B; HEP3B

Species

Human

Cat.No

ABC-TC5585

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Epithelial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Liver

Disease

Hepatocellular Cancer

Storage

Liquid Nitrogen

Product Type

Human Liver Cancer Cell Lines

Description

Hep 3B is a human hepatoma cell line originally derived from liver tissue obtained from an 8-year-old Black patient with liver cancer.Hep 3BHep 3B, also known as Hep 3B2.1-7, is a human hepatocellular carcinoma (HCC) cell line derived from the liver tissue of an 8-year-old Black male patient diagnosed with liver cancer. These cells exhibits epithelial-like morphology and display typical adherent culture properties in vitro. Crytogenetically, Hep3B cells possess an aneuploid karyotype with an average chromosomal count of 60. Notably, Hep 3B harbors an integrated hepatitis B virus (HBV) genome and expresses high levels of hepatocyte-associated markers including alpha-fetoprotein (AFP), hepatitis B surface antigen (HBsAg), albumin, and transferrin protein. Hep 3B is tumorigenic, and capable to form tumors in immunodeficient (nude) mice, undercoring its utility in cancer modeling.

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Citation

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Application

  • Hep 3B cell line are a valuable resource for a broad spectrum of hepatology and oncology research applications. They serve as a robust in vitro model for studying the molecular mechanisms of HBV-associated hepatocarcinogenesis, enabling researchers to explore the interplay between viral integration and tumor progression. Additionally, these cells is widely used in drug metabolism, hepatotoxicity studies, and the analysis of plasma protein biosynthesis, particularly in relation to liver-specific functions. Its tumorigenic properties and stable expression of liver-specific markers make these cells an indispensable platform for developing anticancer therapies, antiviral strategies, and understanding liver pathophysiology.

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