For research use only
| Cat No. | ABC-TC0364 |
| Product Type | Human Uterine Cancer Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Cervix |
| Disease | Cervix Cancer |
| Product Code | Hep-2; HEP-2; Hep2 (HeLa derivative); HEp-2/HeLa; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2 |
Hep2 Cervix Carcinoma Cells originate from human laryngeal tumors and are useful in studying epithelial cancer biology and therapeutic responses.
Hep 2 cells (HEp-2) are human epithelial cells that were originally described as being derived from a laryngeal carcinoma but were subsequently identified as a HeLa derivative resulting from cross-contamination. The cells exhibit an adherent epithelial morphology and are keratin-positive, with HeLa marker chromosomes and human papillomavirus (HPV) DNA sequences detected in characterization studies. Because of their established cellular characteristics and extensive historical use, Hep 2 cells remain an important research model for immunofluorescence-based autoantibody detection, virology, host-pathogen interaction studies, and epithelial cell biology.
| Product Code | Hep-2; HEP-2; Hep2 (HeLa derivative); HEp-2/HeLa; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2 |
| Species | Human |
| Cat.No | ABC-TC0364 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Cervix |
| Disease | Cervix Cancer |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Uterine Cancer Cell Lines |
Hep 2 cells are widely used as a cellular substrate for indirect immunofluorescence assays to detect and characterize autoantibodies. HEp-2 substrates provide abundant intracellular antigens and distinct nuclear, cytoplasmic, and mitotic structures that allow visualization of antibody-binding patterns. The cells can support research involving antinuclear antibody (ANA) detection, autoantibody pattern analysis, immunofluorescence assay development, and autoimmune disease research. Hep 2 cells are also used in virology and host-pathogen interaction studies, including research involving viral infection, cellular responses, and antiviral activity.
Research using Hep 2 cells focuses particularly on autoantibody detection, indirect immunofluorescence, ANA pattern recognition, autoimmune disease research, and host-pathogen interactions. HEp-2 cell substrates can reveal antibody reactivity against nuclear, cytoplasmic, and mitotic cellular structures, making them useful for characterizing different autoantibody staining patterns. The cell line has also been used in virological research because of its susceptibility to selected human viruses and its well-characterized epithelial phenotype. When interpreting experimental results, researchers should consider the documented HeLa-derived identity of HEp-2 rather than treating the cells as an authentic laryngeal carcinoma model.
Cell Recovery(Thaw and recover the cryopreserved Hep 2 cells under appropriate culture conditions.) → Cell Culture(Maintain the adherent epithelial cells under the recommended culture conditions.) → Cell Expansion(Expand the cells to obtain sufficient material for downstream experiments.) → Experimental Treatment(Expose cells to patient sera, antibodies, viral preparations, candidate compounds, or other experimental conditions according to the study design.) → Immunofluorescence Staining(Apply primary sera or antibodies and appropriate detection reagents to visualize cellular antigen recognition.) → Signal Detection(Observe and record fluorescence signals and cellular staining patterns using fluorescence microscopy.) → Pattern Analysis(Evaluate nuclear, cytoplasmic, or mitotic staining patterns and compare results with appropriate controls.) → Data Interpretation(Integrate staining patterns and experimental results for autoantibody characterization, infection studies, or other research applications.)
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
