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Primary Cells

Human Amniotic Epithelial Cells

  • For research use only

Cat No.

ABC-TC3486
Product Type

Female Reproductive Cells
Cell Type

Epithelial
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Amniotic
Disease

Normal
Storage

Liquid Nitrogen

HAEpiC from AcceGen Research Laboratories are isolated from human amniotic membranes.

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  • Human Amniotic Epithelial Cells
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Home | Primary Cells | Human Primary Cells | Reproductive System Primary Cells | Human Amniotic Epithelial Cells

Description

Human amniotic epithelial cells (hAECs) are isolated from the innermost layer of the term placenta closest to the fetus. Following primary culture, these cells are cryopreserved. They exhibit a characteristic cobblestone epithelial morphology and grow as adherent monolayers. Evidence supported by animal studies has demonstrated the benefits of amniotic epithelial cells. hAECs show biological activities associated with multiple physiological and pathological processes, including neurological disorders, lung and liver injury, diabetes, acute kidney failure, cardiovascular diseases, wound healing, stage III pressure ulcer repair, intrauterine adhesion, and premature ovarian failure (POF). Isolated hAECs express surface markers of embryonic stem cells including stage-specific embryonic antigen-3 and 4 (SSEA-3 and SSEA-4), octamer-binding transcription factor-4 (OCT-4), and tumor rejection antigen 1-60 and 1-81 (TRA-1-60 and TRA-1-81). Although hAECs possess stem cell-like characteristics, they do not exhibit unlimited proliferation due to the absence of telomerase activity and pose no risk of tumorigenesis in vivo. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.

Product Code

Human Amniotic Epithelial Cells, Amnion Epithelial Cells Human, HAEpiCs, Human Amnion Derived Epithelial Cells, Amniotic Membrane Epithelial Cells
Species

Human
Cat.No

ABC-TC3486
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Amniotic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Female Reproductive Cells
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • Human Amniotic Epithelial Cells provide a powerful platform for investigating regenerative medicine applications, particularly in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF) and liver cirrhosis. Their ability to modulate TGF-β1/Smad3 and promote M2 macrophage polarization makes them ideal for studying anti-fibrotic mechanisms in IPF and liver cirrhosis. These cells enable evaluation of paracrine-mediated antifibrotic activity.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • Can Human Amniotic Epithelial Cells (HAECs) be expanded, and how many passages are recommended?

    HAECs can be expanded for a limited number of passages, typically 3–5 generations under AcceGen’s recommended culture conditions. However, it is recommended that customers use them at a lower cell generation number to ensure that the cells can show a better state in the experiment.

  • Do they form basal-apical polarity when grown in standard plasticware?

    HAECs can be cultured in three-dimensional systems using matrices such as Matrigel, collagen I, or decellularized amniotic membrane matrices. This approach enhances polarity and functions closer to the in vivo state. When cultured in regular plasticware, HAECs may form partial polarity, but not a fully developed basal-apical polarity. Culturing them on Transwell membranes or matrix-coated surfaces can significantly improve structural polarity.

  • What are the donor characteristics of Human Amniotic Epithelial Cells?

    HAECs are exclusively derived from female donors, as they are obtained from post-partum placental tissue. Immunofluorescence characterization confirms strong PCK expression, with a purity above 90%.

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