Human Aortic Adventitia Fibroblasts

Description

Human Aortic Adventitia Fibroblasts are primary cells isolated from the tunica externa of human ascending or descending aorta. Following primary culture, these cells are cryopreserved. These cells exhibit a characteristic spindle-shaped morphology and grow as adherent monolayers. These cells are widely utilized to investigate the role of fibroblasts in aortic diseases, as they play critical roles in vascular remodeling through collagen/elastin synthesis and cytokine secretion (e.g., TGF-β, IL-6). Their pathological activation contributes to fibrosis in aortic adventitia, aortic aneurysms, atherosclerosis progression, and vascular stiffening in hypertension. Human Aortic Adventitia Fibroblasts express fibroblast markers vimentin and collagen I. To preserve optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.