Human Cord Blood CD14+ Monocytes

Description

Human Cord Blood CD14⁺ Monocytes are primary immune cells isolated from healthy human umbilical cord blood, providing a valuable source of naïve monocytes for developmental immunology research. Morphologically, these cells are large, round or oval-shaped mononuclear cells with characteristic CD14⁺CD16⁻ (classical) and CD14⁺CD16⁺ (intermediate) surface marker expression, maintaining high purity through optimized isolation protocols. These non-proliferative cells are precursors to macrophages and dendritic cells, capable of phagocytosis, antigen presentation, and cytokine secretion (e.g., TNF-α, IL-6). The cord blood-derived monocytes display enhanced plasticity, with upregulated genes involved in immune regulation (IL-10, TGF-β) and angiogenesis (VEGF). Their immature phenotype makes them particularly suitable for studying monocyte-to-macrophage differentiation and hematopoietic development. These human CD14⁺ monocytes offer a robust platform for investigating innate immune responses, modeling inflammatory processes, and evaluating therapeutic interventions in early-life or neonatal immunology studies.