Human Dermal Lymphatic Microvascular Endothelial Cells

Description

Human Dermal Lymphatic Microvascular Endothelial Cells (HDLECs) are primary cells isolated from lymphatic capillaries of normal human dermis. Following primary culture, these cells are cryopreserved. These specialized endothelial cells maintain unique lymphatic functions while sharing some characteristics with blood vascular endothelial cells. HDLECs also play vital roles in immune regulation, interstitial fluid transport, and lipid metabolism with their dysfunction linked to pathological conditions including cancer metastasis and lymphedema. Although lymphatic microvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Characterized by positive expression of lymphatic-specific markers PROX1 and VEGFR3 (FLT4) and negative for blood endothelial marker CD34, these cells provide an authentic model for studying lymphatic biology. To maintain optimal performance, repeated freezing and thawing should be avoided during culture, particularly for lymphatic permeability studies. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.