Human Fibroblast-Like Synoviocyte Normal Tissue, Adult

Human Fibroblast-Like Synoviocytes (HFLS) are primary cells isolated from normal adult human joint synovial membranes, exhibiting fibroblast-like, spindle-shaped morphology and adherent growth patterns. They are cryopreserved at eraly passage. Functionally, HFLS produce extracellular matrix molecules like hyaluronic acid, lubricin, and glycoproteins, crucial for joint lubrication and integrity. They help maintain synovial membrane structure and support mechanical movement. Under pathological stimuli like rheumatoid arthritis (RA), they become aggressive, secreting IL-6, IL-8, and MMPs, contributing to synovial inflammation and pannus formation. Gene expression profiling confirms high levels of CD90, vimentin, and fibronectin, typical for mesenchymal cells. HFLS do not exhibit tumorigenicity.