Human Metabolic Gene Knockout Library Cells

Description

Human Metabolic Gene Knockout Library Cells are a pool of human cells with CRISPR-Cas9‑mediated knockout of genes involved in metabolism, including enzymes and transporters for carbohydrate, lipid, amino acid, and nucleotide pathways. Each target gene is disrupted by multiple independent sgRNAs. The stable pooled library is cryopreserved and delivered at ≥300× cell coverage, with >99% gRNA representation and uniformity <10. We provide frozen cells shipped on dry ice. Upon receipt, cells must be transferred to liquid nitrogen or stored at –80°C for up to 24 hours, or thawed immediately for direct recovery.

Species	Human
Cat.No	ABC-LC0004Y
Product Category	Library cells
Size/Quantity	1 vial
Shipping Info	Dry Ice
Growth Conditions	37 ℃, 5% CO₂
Biosafety Level	1
Storage	Liquid Nitrogen
Product Type	Library Cells
Host Cell	Human cell line background (varies by product configuration)

Quality Control

For library cell pools, sgRNA coverage must be assessed via NGS sequencing, with a general acceptance criterion of >90% coverage. Furthermore, AcceGen performs rigorous quality control on all library cell pools, including mycoplasma testing, bacterial contamination screening, and cell post-recovery viability validation, to guarantee high-quality product delivery.

Application