Human Whole-Genome CRISPR Interference Library Cells

Description

Human Whole-Genome CRISPR Interference Library Cells are a pool of human cells stably expressing dCas9 fused to a transcriptional repressor domain. The library contains sgRNAs targeting the transcription start sites of protein‑coding genes to enable reversible, partial knockdown of gene expression. Each target gene is targeted by multiple independent sgRNAs. The stable pooled library is cryopreserved and delivered at ≥300× cell coverage, with >99% gRNA representation and uniformity <10. We provide frozen cells shipped on dry ice. Upon receipt, cells must be transferred to liquid nitrogen or stored at –80°C for up to 24 hours, or thawed immediately for direct recovery.

Species	Human
Cat.No	ABC-LC0008Y
Product Category	Library cells
Size/Quantity	1 vial
Shipping Info	Dry Ice
Growth Conditions	37 ℃, 5% CO₂
Biosafety Level	1
Storage	Liquid Nitrogen
Product Type	Library Cells
Host Cell	Human cell line background (varies by product configuration)

Quality Control

For library cell pools, sgRNA coverage must be assessed via NGS sequencing, with a general acceptance criterion of >90% coverage. Furthermore, AcceGen performs rigorous quality control on all library cell pools, including mycoplasma testing, bacterial contamination screening, and cell post-recovery viability validation, to guarantee high-quality product delivery.

Application