Human Ovarian Microvascular Endothelial Cells

Description

Human Ovarian Microvascular Endothelial Cells (HOMECs) are primary endothelial cells derived from the microvasculature of adult human ovarian tissue and cryopreserved after primary culture. These cells display characteristic cobblestone morphology and express key endothelial markers including CD31, VE-cadherin, Ulex europaeus agglutinin I (UEA-1), and von Willebrand factor (vWF), supporting their role in maintaining the ovary microvascular barrier and endothelial integrity. HOMECs also express VCAM-1, reflecting an activated endothelial phenotype relevant to inflammatory and pathological conditions. Molecular profiling by RT-PCR confirms expression of VEGF receptors (flt-1, KDR) and endothelial nitric oxide synthase (eNOS), highlighting their involvement in ovarian blood vessel formation and vascular tone regulation through coordinated ovary endothelial cell signaling pathways. Functionally, these cells regulate selective permeability, angiogenesis, and immune cell trafficking within ovarian tissue and provide a physiologically relevant model for studying ovarian angiogenesis, inflammation, and reproductive pathologies. In disease-oriented research, HOMECs are also applicable to investigations of endothelial alterations associated with ovarian tumors, aligning with studies of ovarian tumor endothelial cells. To preserve cellular integrity and functional performance, repeated freeze–thaw cycles should be avoided during in vitro handling.