Human Ovarian Surface Epithelial Cells (HOSECs) are primary cells isolated from the outer layer of healthy human ovaries. In vivo, these cells display diverse morphologies—squamous, cuboidal, and low columnar—and play a crucial role in ovulatory rupture and tissue repair. In culture, they form a cobblestone-like morphology and adhere strongly to surfaces. HOSECs express key markers like CK7, E-cadherin, and estrogen/progesterone receptors, verified by immunostaining. When co-cultured with endometrial stromal cells under estrogen-rich conditions, these cells form gland-like structures. These cells are early passage, non-tumorigenic, and widely utilized in research on ovarian cancer, epithelial-to-mesenchymal transition (EMT), and tissue regeneration. They offer insights into ovarian function and pathology, supporting both basic and translational studies.
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