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Primary Cells

Human Pancreatic Epithelial Cells

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Human Pancreatic Epithelial Cell were initiated by elutriation of dispase dissociated normal pancreatic tissue. These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in […]
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Home | Primary Cells | Human Primary Cells | Endocrine System Primary Cells | Human Pancreatic Epithelial Cells
Species

Human
Cat.No

ABC-TC3744
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Pancreas
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Pancreatic Cells

Description

Human Pancreatic Epithelial CellsHuman Pancreatic Epithelial Cells are isolated from healthy pancreatic tissue and constitute a vital component of the mature pancreas, which features an intricate, branching tubular epithelial network predominantly serving exocrine functions. As the pancreatic epithelium differentiates during late gestation, acinar cells mature at the branch tips, while ductal and endocrine progenitors emerge in the branch stalks. Through delamination, endocrine cells break away from their neighboring epithelial cells and come together to form islets. Simultaneously, the remaining tubular epithelium gives rise to ductal branches. This coordinated growth from a common pre-pancreatic epithelium results in the harmonious integration of both exocrine and endocrine compartments, forming a dual-function organ.

 

Why choose Human Pancreatic Epithelial Cells from AcceGen?

Human Primary Pancreatic Epithelial Cells from AcceGen are isolated from human pancreatic tissue. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5×10^6 cells per ml and is delivered frozen.

AcceGen Frozen Cells & Cell Lines 1 vial
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Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • FOR RESEARCH USE ONLY

  • Human Pancreatic Epithelial Cells find valuable applications in scientific research, particularly in investigating the functional impact of activating point mutations in the K-Ras oncogene, which are prevalent in pancreatic cancer and play a crucial role in its early development. These cells serve as an in vitro model to study the transformation of normal pancreatic epithelial cells into cancerous ones. Additionally, they are utilized to identify potential human pancreatic carcinogens by subjecting them to various factors for exposure analysis.

Frequently Asked Questions

  • What are Human Pancreatic Epithelial Cells, and how are they obtained?

    Human Pancreatic Epithelial Cells are cells derived from the epithelial tissue of the pancreas. They are typically isolated from donor pancreatic tissue using enzymatic digestion and are cultured for research purposes.

  • What are the primary research applications for Human Pancreatic Epithelial Cells?

    These cells are commonly used in studies related to pancreatic diseases, including diabetes, pancreatic cancer, and pancreatitis. They are also used in drug testing, toxicology studies, and research on pancreatic function and regeneration.

  • How should Human Pancreatic Epithelial Cells be cultured?

    Human Pancreatic Epithelial Cells should be cultured in a specialized epithelial cell medium that supports their growth and function. The use of pre-coated surface may also be necessary to promote cell adhesion and proliferation.

  • What are the key differences between Human Pancreatic Epithelial Cells and islet cells?

    Human Pancreatic Epithelial Cells are primarily involved in the formation of ducts and the secretion of digestive enzymes, whereas islet cells (including beta cells) are responsible for hormone production, such as insulin. Each cell type has distinct roles and is used for different types of research.

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