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Primary Cells

Human Preadipocytes-Visceral

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Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissue and can proliferate and differentiate into mature adipocytes according […]
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Home | Primary Cells | Human Primary Cells | Endocrine System Primary Cells | Human Preadipocytes-Visceral
Product Code

HPA-v
Species

Human
Cat.No

ABC-TC3759
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Preadipocyte
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Visceral
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Adipose Cells/Preadipocytes

Description

Human Preadipocytes-Visceral
Human preadipocytes-visceral (HPA-v) are derived from human visceral adipose tissue. Following the primary culture, these cells are cryopreserved. HPA-v are useful for studying adipose tissue development, function, and regulation. After differentiation, HPA-v are characterized using CD44, CD90 antibodies and lipid staining for immunofluorescence. HPA-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. These cells should be avoided repeated freezing and thawing during the culture process.

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Citation

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Application

  • HPA-v cells are precursor cells from visceral adipose tissue and are widely used in studies of adipogenesis, obesity, and metabolic disorders. They offer a physiologically relevant human model for exploring the cellular mechanisms of fat development, lipid metabolism, and the effects of drugs and nutritional factors. This cell line also aids in modeling diseases such as diabetes and metabolic syndrome, providing insights into the role of visceral fat in systemic health.

Frequently Asked Questions

  • Can the pre-adipocytes be cultured without using poly-L-lysine (PLL)?

    Yes, the pre-adipocytes can be cultured without using poly-L-lysine (PLL). However, when the cells are first thawed, their attachment rate may be lower. It is recommended to let the cells rest for 18-36 hours to allow sufficient time for attachment.

  • Are Human Preadipocytes-Visceral derived from stromal vascular fractions of visceral fat, and how were Human Preadipocytes-Visceral identified and validated for purity?

    Human Preadipocytes-Visceral are isolated from stromal vascular fractions of visceral fat and represent preadipocytes/adipose-derived stem cells, which can subsequently differentiate into mature adipocytes. Since mature adipocytes are non-proliferative, preadipocytes are the proliferative population that can be expanded and studied in vitro. Cell identification is confirmed by Oil Red O staining, with a measured purity of over 90%.

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