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Primary Cells

Human Preadipocytes-Visceral

  • For research use only

Cat No.

ABC-TC3759
Product Type

Adipose Cells/Preadipocytes
Cell Type

Preadipocyte
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Visceral
Disease

Normal
Storage

Liquid Nitrogen

Human visceral preadipocytes for adipose research, differentiation studies, and immunofluorescence. Cryopreserved and pathogen-free.

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  • Human Preadipocytes-Visceral
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Home | Primary Cells | Human Primary Cells | Endocrine System Primary Cells | Human Preadipocytes-Visceral

Description

Human Preadipocytes–Visceral (HPA-v) are progenitor cells isolated from visceral adipose depots (e.g., omental or mesenteric fat) of healthy donors and cryopreserved after primary culture, and are widely referenced as preadipocytes in human adipose biology studies, often contrasted with non-human models such as rhesus monkey preadipocytes in comparative metabolic research. These spindle-shaped, fibroblast-like cells exhibit high proliferative capacity and serve as precursors to mature adipocytes. They express markers such as CD34, Pref-1 (DLK1), and PPARγ, and can differentiate into lipid-accumulating adipocytes under adipogenic conditions. Visceral preadipocytes possess unique metabolic characteristics distinct from their subcutaneous counterparts, including a stronger association with insulin resistance, chronic inflammation, and metabolic disorders such as type 2 diabetes and cardiovascular disease. HPA-v cells are a valuable in vitro model for studying depot-specific adipogenesis, adipokine signaling, and the pathogenic roles of visceral fat in obesity-related diseases.

Product Code

HPA-v
Species

Human
Cat.No

ABC-TC3759
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Preadipocyte
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Visceral
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Adipose Cells/Preadipocytes
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • HPA-v cells are precursor cells from visceral adipose tissue and are widely used in studies of adipogenesis, obesity, and metabolic disorders. They offer a physiologically relevant human model for exploring the cellular mechanisms of fat development, lipid metabolism, and the effects of drugs and nutritional factors. This cell line also aids in modeling diseases such as diabetes and metabolic syndrome, providing insights into the role of visceral fat in systemic health.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • Can the pre-adipocytes be cultured without using poly-L-lysine (PLL)?

    Yes, the pre-adipocytes can be cultured without using poly-L-lysine (PLL). However, when the cells are first thawed, their attachment rate may be lower. It is recommended to let the cells rest for 18-36 hours to allow sufficient time for attachment.

  • Are Human Preadipocytes-Visceral derived from stromal vascular fractions of visceral fat, and how were Human Preadipocytes-Visceral identified and validated for purity?

    Human Preadipocytes-Visceral are isolated from stromal vascular fractions of visceral fat and represent preadipocytes/adipose-derived stem cells, which can subsequently differentiate into mature adipocytes. Since mature adipocytes are non-proliferative, preadipocytes are the proliferative population that can be expanded and studied in vitro. Cell identification is confirmed by Oil Red O staining, with a measured purity of over 90%.

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