Human Whole Aortic Cells

Description

Human Whole Aortic Cells encompass a heterogeneous population of cells derived from the aortic wall, including endothelial cells (ECs), smooth muscle cells (SMCs), fibroblasts, and vascular progenitor cells. These cells originate from the aortic tunica intima (endothelial layer), media (smooth muscle layer), and adventitia (connective tissue layer). In culture, the mixed cell population may display diverse morphologies reflecting the presence of different vascular cell types, including polygonal endothelial-like cells, spindle-shaped smooth muscle–like cells, and flattened fibroblast-like cells. Functionally, ECs regulate vascular tone and barrier function, SMCs contribute to vascular contractility and extracellular matrix remodeling, while fibroblasts support connective tissue structure through collagen production. Key molecular markers include CD31 and von Willebrand Factor (vWF) for ECs, alpha-smooth muscle actin (α-SMA) and SM22α for SMCs, and fibroblast-specific protein-1 (FSP1)/S100A4 for fibroblasts. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.