Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (hMSCs) are usually derived from bone marrow samples of healthy volunteers. These cells show a typical spindle-shaped morphology and adhere well in culture. Immortalized using retroviral SV40 large T antigen, they exhibit robust proliferation without senescence. These hMSCs express standard mesenchymal markers including CD73, CD90, and CD105, and are negative for CD34, CD45, and HLA-DR. Functionally, they differentiate into osteogenic, adipogenic, and chondrogenic lineages and support primitive hematopoietic cell growth. Non-tumorigenic and non-metastatic, they are ideal for long-term in vitro applications such as stem cell biology, tissue engineering, and hematopoietic niche modeling. Recommended for use under biosafety level 2 (BSL-2) conditions.
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