Immortalized Human Fibroblast-Like Synoviocyte Rheumatoid Arthritis

Description

Immortalized Human Fibroblast-Like Synoviocyte (FLS) Rheumatoid Arthritis (RA) is an immortalized cell line derived from synovial tissue of an RA patient, belonging to disease-specific human fibroblast cell lines. These spindle-shaped fibroblast-like cells grow adherently and retain key phenotypic traits of primary RA FLS, including expression of vimentin, fibronectin, and pro-inflammatory mediators such as IL-6, IL-8, and MMP-3. The cell line exhibits a complex, abnormal karyotype with chromosomal instability, mirroring primary RA synoviocytes. Immortalization enables continuous culture while preserving pathological features relevant to RA, including hyperproliferation and invasiveness that drive joint destruction. Widely used as an in vitro model, these cells facilitate studies on pannus formation, extracellular matrix degradation, and cytokine-mediated inflammation in RA pathogenesis.

Application

• Immortalized Human RA FLS are extensively utilized for in vitro studies of RA pathogenesis, including synovial inflammation, matrix degradation, and immune cell interactions. They serve as a reliable platform for screening anti-inflammatory drugs, biological agents, and signaling pathway inhibitors targeting synovial hyperplasia and joint destruction. These cells also facilitate mechanistic studies on cell migration, adhesion, and cytokine production under inflammatory conditions.