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INS-1

Product Name

INS-1

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Product Code

INS1

Cat.No

ABC-TC232S

Species

Rattus Norvegicus

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

The INS-1 cell line, derived from x-ray-induced insulinoma in rats, exhibits a high insulin content and responsiveness to glucose changes. Cultivation relies on 2-mercaptoethanol and showcases gradual proliferation (100-hour doubling time) with beta-cell-like morphology. Heterogeneous in nature, INS-1 cells comprise mature insulin-positive and immature bi-hormonal cells co-expressing insulin and glucagon. Bi-hormonal variants, lower in Nkx6.1 and lacking alpha cell markers, imply incomplete maturation. Prolonged glucose exposure diminishes insulin gene and protein expression, subsequently reducing insulin and proglucagon-derived peptide levels, including GLP-1, GLP-2, and glucagon.

 

Why choose INS-1 from AcceGen?

INS-1 cells exhibit superior viability and quality, maintained under sterile conditions with optimal incubation settings. These cells are cryopreserved using advanced techniques and undergo rigorous quality control, ensuring their reliability for various experimental applications.

Source Organ

Pancreas

Recommended Medium And Supplement
Citation Guide

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Application

FOR RESEARCH USE ONLY

The INS-1 cell line, renowned for its stability and preserved differentiation, serves as an ideal model to investigate diverse facets of beta-cell activity. It finds extensive utility in deciphering insulin secretion mechanisms, particularly excelling as a tool to explore glucose-triggered insulin release. Moreover, INS-1 cells offer valuable insights into the expression and regulation of proglucagon-derived peptides within primary islets, unraveling their responses to a range of metabolic stimuli.

 

Growth Conditions

37 ℃, 5% CO2

Cell Type

β Cell

Growth Mode

Adherent

Product Type

Rat Pancreas Cancer Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What are INS-1 cells?

    The INS-1 cell line is an immortalized cells induced by radiation, isolated from pancreas of an x-ray-created transplantable insulinoma rats.

  • What culture medium is recommended for INS-1 cells?

    The culture medium recommended for INS- 1 cells is composed of 1640 medium supplemented with 4.5 g/L Glucose, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium pyruvate, 1.5 g/L NaHCO3 to support optimal cell growth and maintenance, add 10% FBS.
    The sodium pyruvate, HEPES, and mercaptoethanol should be added at the specified concentration for cell growth.

  • Why are my INS-1 cells do not attach?

    The INS-1 cell line show weak attachment at the beginning of the recovery, usually take 24-48 hours without changing medium. In the first few days, observing floating cells is normal. This cell also grow slowly, may form attached clumps. Please be patient and take care. Normally 3 days after thawing, the cells can be trypsinized and plated on 2 flasks to expand.

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