NCI-H460

NCI-H460 is a human large cell lung carcinoma cell line established in 1982 from the pleural effusion of a 42-year-old African-American male patient with metastatic disease. NCI-H460 exhibits epithelial-like morphology and adherent growth in monolayer culture. These cells have a hypotriploid karyotype, with a modal chromosome number of 57 (range 53–65), and a doubling time of approximately 23–33 hours. These cells express keratin and vimentin, indicative of both epithelial and mesenchymal phenotypes, but is negative for neurofilament triplet proteins. NCI-H460 demonstrates tumorigenic potential in nude mice models, forming subcutaneous xenografts.