OCI-AML3

Frequently Asked Questions

• What is the culture condition of OCI-AML3 Cell line?

  1. The recommended culture medium for OCI-AML3 is RPMI 1640 + 20%FBS.
  Preheat the complete culture medium in a 37℃ water bath for 30 minutes.
  Use a pipette to draw 6-7 mL of complete culture medium into a 15 mL centrifuge tube in a clean bench.

  2. Then take the frozen cells out of the liquid nitrogen or dry ice. When reviving, shake them slightly to remove the residual dry ice or liquid nitrogen, then quickly clamp the lid with tweezers and place it in a 37℃ water bath and shake it quickly (note: the water cannot cover the lid), so that it completely melts in about 1 minute.

  3. In the clean bench, wipe the outer wall of the cryotube with an 75% alcohol to disinfect it and let it dry slightly. Use a single-channel pipette to transfer all thawed cell suspensions to the complete culture medium prepared in advance, cover the lid, and centrifuge at 1100rpm for 4 minutes at room temperature to collect cells.

  4. Carefully aspirate the supernatant in the clean bench, use a single-channel pipette to absorb 1 mL of fresh complete culture medium to resuspend the cells into a single cell suspension, and then transfer to a T25 culture flask (or 6cm dish) containing 5 mL of complete culture medium.

  Note: Do not directly recover to a T75 flask or a 10cm dish.

• How does OCI-AML3 compare to other AML cell lines?

  OCI-AML3 was established from the peripheral blood of a 57-year-old man diagnosed with acute myeloid leukemia (AML). The cells have specific genetic mutations that are representative of AML, carrying both NPM1 gene mutation and the DNMT3A R882C mutation. The NPM1 gene mutation is of type A, which refers to mutations that involve the nucleophosmin 1 (NPM1) gene.

• What is the doubling time of OCI-AML3 cell line?

  The doubling time of OCI-AML3 cells is approximately 28-40 hours, though this can vary depending on culture conditions.