Rat Hepatic Stellate Cells

Description

Rat Hepatic Stellate Cells (RHSCs) are liver-resident pericytes isolated from the space of Disse in rat hepatic tissue. In their quiescent state, these cells are characterized by their capacity to store vitamin A in cytoplasmic lipid droplets and express markers such as desmin, GFAP, and cellular retinol-binding protein 1 (CRBP-1). Upon liver injury or inflammatory stimulation, RHSCs activate into myofibroblast-like cells, expressing α-smooth muscle actin (α-SMA) and secreting extracellular matrix components. This transdifferentiation plays a central role in hepatic fibrogenesis. To ensure experimental consistency, repeated freeze-thaw cycles should be avoided. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.