For research use only
| Cat No. | ABC-TC4136 |
| Product Type | Rat Primary Cells |
| Cell Type | Hepatocyte |
| Species | Rat |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Normal |
| Storage | Liquid Nitrogen |
Rat Hepatocytes support liver research by providing a functional primary hepatocyte model for drug metabolism, toxicity testing, and hepatic biology.
Rat hepatocytes are primary liver cells isolated from rat liver and are widely used to investigate hepatic physiology, xenobiotic metabolism, and liver toxicity. Hepatocytes perform essential functions, including drug biotransformation, nutrient metabolism, and the synthesis and secretion of liver-associated proteins. Primary rat hepatocytes provide an established in vitro model for evaluating cytochrome P450 (CYP450) enzyme activity, drug metabolism, and cellular responses to chemical exposure. Their metabolic characteristics can change during in vitro culture, making culture conditions and experimental timing important considerations when assessing hepatic function.
| Product Code | RH |
| Species | Rat |
| Cat.No | ABC-TC4136 |
| Product Category | Primary Cells |
| Size/Quantity | 1 vial |
| Cell Type | Hepatocyte |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Rat Primary Cells |
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
Rat hepatocytes can be used in drug metabolism studies, hepatotoxicity screening, CYP450 enzyme activity assays, drug–drug interaction research, and investigations of xenobiotic-induced enzyme regulation. Studies have evaluated CYP isoform activities and enzyme inducibility in primary rat hepatocytes cultured under different extracellular matrix configurations. Separate studies have investigated phase II metabolism in primary cultures of adult rat hepatocytes, including the metabolism of warfarin. These models can support the evaluation of compound metabolism and treatment-associated changes in hepatic enzyme activity. Appropriate assay controls and time-matched measurements are important when interpreting metabolic activity and toxicity endpoints.
Rat hepatocytes support research in hepatic drug metabolism, liver toxicology, xenobiotic biotransformation, and metabolic enzyme regulation. CYP450 enzymes contribute to the oxidative metabolism of many compounds, while phase II enzymes participate in conjugation reactions that can facilitate compound elimination. Studies of primary rat hepatocytes have demonstrated that CYP isoform activities and selected phase II metabolic activities can change over time in culture. The direction and magnitude of these changes depend on the enzyme measured and the culture conditions. This model can therefore help researchers investigate compound metabolism and enzyme regulation, provided that culture duration, cell viability, and individual metabolic endpoints are considered during experimental interpretation.
Cell Receipt and Preparation (Handle the rat hepatocytes according to the supplier's instructions and specified product format.) → Cell Seeding (Plate the cells using an appropriate density and culture configuration for the intended assay.) → Cell Attachment and Recovery (Allow cells to recover and attach when applicable to the supplied cell format and culture system.) → Culture Maintenance (Use a validated hepatocyte culture medium and monitor cell morphology and viability.) → Experimental Treatment (Expose the cells to test compounds, enzyme inducers, or other experimental agents.) → Functional Assessment (Measure CYP450 activity, metabolic conversion, cell viability, or other relevant hepatic endpoints.) → Data Analysis (Compare treatment and control groups while accounting for culture duration and assay-specific controls.)
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
