Rat Intestinal Epithelial Cells

Description

Rat Intestinal Epithelial Cells are primary cells isolated from the mucosal layer of the rat small intestine. These cells exhibit a polarized, cobblestone morphology and form tight, adherent monolayers under optimized culture conditions. They firmly adhere to collagen- or fibronectin-coated surfaces and display functional apical-basal polarity typical of intestinal epithelium. Rat Intestinal Epithelial Cells express key markers such as cytokeratin 18, E-cadherin, and ZO-1. The cells also exhibit brush border enzyme activity, including alkaline phosphatase and sucrase-isomaltase, reflecting their absorptive epithelial phenotype. These cells are cryopreserved at early passage to retain high viability and functional integrity. For optimal performance and to maintain cellular integrity, repeated freeze-thaw cycles should be avoided. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.