Rat Lung Fibroblasts-Neonatal

Description

Rat Lung Fibroblasts-Neonatal are primary mesenchymal cells isolated from the lung tissue of neonatal Sprague-Dawley rats. These cells exhibit a classic spindle-shaped morphology and adhere strongly to standard tissue culture surfaces, forming a uniform monolayer during growth. Neonatal lung fibroblasts are particularly responsive to growth factors and mechanical cues due to their developmental stage, making them a valuable in vitro model for studying neonatal lung development and tissue repair. They express mesenchymal markers such as vimentin, fibronectin, and PDGFR-α, and can upregulate α-smooth muscle actin (α-SMA) upon activation. Cryopreserved at low passage, the cells retain high post-thaw viability and consistent morphology. For optimal experimental outcomes, repeated freeze-thaw cycles should be avoided. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.