For research use only
| Cat No. | ABL-TC0743 |
| Product Type | Human Lung Cell Lines |
| Cell Type | Fibroblast |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lung |
| Disease | Normal |
| Product Code | WI-38, WI 38, WI38 Cells, Human Lung Fibroblast Cell Line WI-38, Diploid Fibroblast Cells |
WI-38 ,Human fibroblast-like lung cell line; normal diploid fibroblast with limited lifespan, distributed around 30 PDL for research applications.
WI-38 cells are a human diploid fibroblast cell strain derived from fetal lung tissue and widely used in cellular biology and aging research. These cells have a finite replicative lifespan, making them a well-established model for investigating cellular senescence, growth arrest, and age-associated cellular changes. WI-38 fibroblasts have also been used to study responses to oxidative stress, changes in cellular metabolism, and the effects of culture conditions on cell physiology. Their diploid background and finite lifespan distinguish them from continuously proliferating immortalized cell lines. WI-38 cells may therefore be useful for studies requiring a non-tumor-derived human fibroblast model, including experiments on senescence induction and cellular stress responses. Experimental outcomes can vary with passage number, culture conditions, and the method used to induce senescence, so these factors should be controlled and documented when interpreting results.
| Product Code | WI-38, WI 38, WI38 Cells, Human Lung Fibroblast Cell Line WI-38, Diploid Fibroblast Cells |
| Species | Human |
| Cat.No | ABL-TC0743 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lung |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Lung Cell Lines |
WI-38 cells may be used in studies of cellular senescence, fibroblast biology, oxidative stress, and cell proliferation. Because they have a finite replicative lifespan, researchers can use serial passaging to investigate replicative senescence and associated changes in cell morphology, growth, and molecular markers. WI-38 fibroblasts have also been used to examine how oxygen concentration and growth state affect cellular antioxidant responses. These applications make the cell strain relevant to research on aging-related cellular processes, stress responses, and senescence-associated pathways. WI-38 cells have additionally served as human diploid cell substrates in historical vaccine research and production. For experiments involving senescence or oxidative stress, researchers should establish suitable controls and account for passage history, culture conditions, and treatment exposure. Results from WI-38 cells should not automatically be generalized to all primary human fibroblasts or other cell types.
WI-38 cells are a commonly used model for studying replicative senescence in human diploid fibroblasts. As these cells undergo repeated population doublings, their proliferative capacity changes, allowing researchers to investigate cellular aging and associated molecular responses. Studies using WI-38 fibroblasts have examined the effects of oxygen tension, growth status, and senescence on antioxidant defense systems, providing a framework for investigating how environmental conditions influence cell physiology. Research areas include senescence-associated signaling, oxidative stress, cell-cycle regulation, and age-related changes in cellular function. The model is particularly useful when experimental design requires fibroblasts with a finite lifespan rather than an immortalized cell line. However, senescence-associated characteristics depend on passage number, culture history, and the experimental stimulus; multiple markers and appropriate controls should be used to distinguish senescence from other forms of growth inhibition.
Cell Recovery (Thaw and recover WI-38 cells according to the supplier's recommended protocol.) → Cell Culture (Maintain cells under appropriate culture conditions and monitor morphology and growth.) → Passage Documentation (Record passage number and, where available, population doubling history.) → Experimental Setup (Establish control and treatment groups for senescence, oxidative stress, or other intended studies.) → Treatment or Senescence Induction (Apply the selected experimental conditions using a validated protocol.) → Phenotypic Assessment (Evaluate cell morphology, proliferation, and relevant cellular responses.) → Molecular Analysis (Measure appropriate senescence-associated markers, oxidative stress indicators, or other study-specific endpoints.) → Data Interpretation (Compare results with controls while considering passage history and culture conditions.)
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